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PSMB8 antibody

Anti-PSMB8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02245
Anti-PSMB8 antibody used in Western Blot (WB). GTX02245
Anti-PSMB8 antibody used in IHC (Paraffin sections) (IHC-P). GTX02245
Anti-PSMB8 antibody used in Immunoprecipitation (IP). GTX02245

Cat No. GTX02245

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:300-1:10000
IHC-P 1:500-1:2000
IP 5-10 μl/mg of lysate
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to the C-terminus of the Human PSMB8

Purification

Purified by Protein A and Antigen Affinity

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

proteasome subunit beta 8 , ALDD , D6S216 , D6S216E , JMP , LMP7 , NKJO , PRAAS1 , PSMB5i , RING10

Cellular Localization

Cytoplasm , Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PSMB8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02245

GTX02245 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX02245 PSMB8 antibody.
Green : Primary antibody
Dilution : 1:1000
Permeabilzation : 0.3% Triton X-100

Anti-PSMB8 antibody used in Western Blot (WB). GTX02245

GTX02245 WB Image

WB analysis of various samples using GTX02245 PSMB8 antibody.
Lane A : HeLa whole cell lysate
Lane B : MCF-7 whole cell lysate
Lane C : A431 whole cell lysate
Lane D : Raji whole cell lysate
Dilution : 1:500
Loading : 30 μg

Anti-PSMB8 antibody used in IHC (Paraffin sections) (IHC-P). GTX02245

GTX02245 IHC-P Image

IHC-P analysis of human lymphoma tissue section using GTX02245 PSMB8 antibody.
Dilution : 1:1000

Anti-PSMB8 antibody used in Immunoprecipitation (IP). GTX02245

GTX02245 IP Image

IP analysis of A431 whole cell lysate using GTX02245 PSMB8 antibody.
IP antibody : 2 μl per 0.5 mg total whole cell lysate
Dilution : 1:100
Immunomagnetic beads Protein A/G : 60 μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 339