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PSMC3 antibody [9F3]

Overexpression
Anti-PSMC3 antibody [9F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83778
Anti-PSMC3 antibody [9F3] used in Flow cytometry (FACS). GTX83778
Anti-PSMC3 antibody [9F3] used in IHC (Paraffin sections) (IHC-P). GTX83778
Anti-PSMC3 antibody [9F3] used in IHC (Paraffin sections) (IHC-P). GTX83778
Anti-PSMC3 antibody [9F3] used in Western Blot (WB). GTX83778
Anti-PSMC3 antibody [9F3] used in Flow cytometry (FACS). GTX83778
Anti-PSMC3 antibody [9F3] used in IHC (Paraffin sections) (IHC-P). GTX83778
Anti-PSMC3 antibody [9F3] used in Western Blot (WB). GTX83778
Anti-PSMC3 antibody [9F3] used in IHC (Paraffin sections) (IHC-P). GTX83778

Cat No. GTX83778

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9F3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Dog
Package
100 μl ($389)
See all PSMC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PSMC3(NP_002795) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

proteasome 26S subunit, ATPase 3 , TBP1

Cellular Localization

Cytoplasm,Nucleus

Background

The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation.

Database

Research Area

DATA IMAGES

Anti-PSMC3 antibody [9F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83778

GTX83778 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PSMC3 plasmid using GTX83778 PSMC3 antibody [9F3].

Anti-PSMC3 antibody [9F3] used in Flow cytometry (FACS). GTX83778

GTX83778 FACS Image

FACS analysis of HEK293T cells transfected with either PSMC3 plasmid(Red) or empty vector control plasmid(Blue) using GTX83778 PSMC3 antibody [9F3].

Anti-PSMC3 antibody [9F3] used in IHC (Paraffin sections) (IHC-P). GTX83778

GTX83778 IHC-P Image

IHC-P analysis of bladder carcinoma tissue using GTX83778 PSMC3 antibody [9F3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PSMC3 antibody [9F3] used in IHC (Paraffin sections) (IHC-P). GTX83778

GTX83778 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX83778 PSMC3 antibody [9F3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PSMC3 antibody [9F3] used in Western Blot (WB). GTX83778

GTX83778 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PSMC3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83778 PSMC3 antibody [9F3].
Loading : 5 ug per lane

Anti-PSMC3 antibody [9F3] used in Flow cytometry (FACS). GTX83778

GTX83778 FACS Image

FACS analysis of Jurkat cells using GTX83778 PSMC3 antibody [9F3].
Red : Primary antibody
Blue : Negative control antibody

Anti-PSMC3 antibody [9F3] used in IHC (Paraffin sections) (IHC-P). GTX83778

GTX83778 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83778 PSMC3 antibody [9F3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PSMC3 antibody [9F3] used in Western Blot (WB). GTX83778

GTX83778 WB Image

WB analysis of various cell lines using GTX83778 PSMC3 antibody [9F3].
Loading : 35 ug per lane

Anti-PSMC3 antibody [9F3] used in IHC (Paraffin sections) (IHC-P). GTX83778

GTX83778 IHC-P Image

IHC-P analysis of prostate tissue using GTX83778 PSMC3 antibody [9F3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 389