Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSMC3 antibody [N1C2] (GTX109605) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: MOLT4 (GTX27912)
10% SDS PAGE
GTX109605 diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using PSMC3(GTX109605) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunofluorescence analysis of paraformaldehyde-fixed A549, using PSMC3(GTX109605) antibody at 1:200 dilution.

Immunofluorescent image of a zebrafish embryo eye section using PSMC3 antibody [N1C2] (GTX109605) at a 1:200 dilution. Psmc3 (Green) Actin(Red)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)