APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Molt-4
Predict Reactivity
Mouse, Rat, Bovine, Chicken, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PSMC3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951513
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
proteasome 26S subunit, ATPase 3 , TBP1
Cellular Localization
Cytoplasm (Potential) , Nucleus
Background
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-PSMC3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109605](/templates/default/js/lightbox/images/loading.gif "Anti-PSMC3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109605")
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GTX109605 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PSMC3(GTX109605) antibody at 1:200 dilution.
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GTX109605 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using PSMC3(GTX109605) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX109605 WB Image
Sample (30 ug of whole cell lysate)
A: MOLT4 (GTX27912)
10% SDS PAGE
GTX109605 diluted at 1:5000
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GTX109605 IHC-Wm Image
Immunofluorescent image of a zebrafish embryo eye section using PSMC3 antibody [N1C2] (GTX109605) at a 1:200 dilution. Psmc3 (Green) Actin(Red)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)
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REFERENCE
There are currently no references for PSMC3 antibody [N1C2] (GTX109605). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PSMC3 antibody [N1C2] (GTX109605). Be the first to share your experience with this product.
![Anti-PSMC3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109605](/upload/website/prouct_img/small/GTX109605/GTX109605_IFA_w_23060500_123.webp "Anti-PSMC3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109605")
![Anti-PSMC3 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX109605](/upload/website/prouct_img/small/GTX109605/GTX109605_IHC_w_23060500_513.webp "Anti-PSMC3 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX109605")
![Anti-PSMC3 antibody [N1C2] used in Western Blot (WB). GTX109605](/upload/website/prouct_img/small/GTX109605/GTX109605_WB_w_23060500_402.webp "Anti-PSMC3 antibody [N1C2] used in Western Blot (WB). GTX109605")
![Anti-PSMC3 antibody [N1C2] used in IHC (Whole mount) (IHC-Wm). GTX109605](/upload/website/prouct_img/small/GTX109605/GTX109605_40023_IHC-Wm_Z_22111423_320.webp "Anti-PSMC3 antibody [N1C2] used in IHC (Whole mount) (IHC-Wm). GTX109605")
![Anti-PSMC3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109605](/upload/website/prouct_img/normal/GTX109605/GTX109605_IFA_w_23060500_123.webp "Anti-PSMC3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109605")
![Anti-PSMC3 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX109605](/upload/website/prouct_img/normal/GTX109605/GTX109605_IHC_w_23060500_513.webp "Anti-PSMC3 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX109605")
![Anti-PSMC3 antibody [N1C2] used in Western Blot (WB). GTX109605](/upload/website/prouct_img/normal/GTX109605/GTX109605_WB_w_23060500_402.webp "Anti-PSMC3 antibody [N1C2] used in Western Blot (WB). GTX109605")
![Anti-PSMC3 antibody [N1C2] used in IHC (Whole mount) (IHC-Wm). GTX109605](/upload/website/prouct_img/normal/GTX109605/GTX109605_40023_IHC-Wm_Z_22111423_320.webp "Anti-PSMC3 antibody [N1C2] used in IHC (Whole mount) (IHC-Wm). GTX109605")