APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.71 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PSMC4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10620613
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
proteasome 26S subunit, ATPase 4 , MIP224 , RPT3 , S6 , TBP-7 , TBP7
Cellular Localization
Cytoplasm , Nucleus
Background
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with an orphan member of the nuclear hormone receptor superfamily highly expressed in liver, and with gankyrin, a liver oncoprotein. Two transcript variants encoding different isoforms have been identified. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX114675 WB Image
Sample (30 ug of whole cell lysate)
A: JurKat
10% SDS PAGE
GTX114675 diluted at 1:1000
|
|
|
GTX114675 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A431, using PSMC4(GTX114675) antibody at 1:200 dilution.
|
|
|
GTX114675 IHC-P Image
PSMC4 antibody detects PSMC4 protein at cytosol and nucleus on Mahlarvu xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Mahlarvu xenograft.
PSMC4 antibody (GTX114675) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
REFERENCE
There are currently no references for PSMC4 antibody (GTX114675). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PSMC4 antibody (GTX114675). Be the first to share your experience with this product.
