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PSMC5 antibody [25D5]

Anti-PSMC5 antibody [25D5] used in Western Blot (WB). GTX70288
Anti-PSMC5 antibody [25D5] used in Western Blot (WB). GTX70288

Cat. No. GTX70288

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 25D5

Isotype

 IgG1

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all PSMC5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encoding full-length SUG1 purified from E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_373070

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome 26S subunit, ATPase 5 , S8 , SUG-1 , SUG1 , TBP10 , TRIP1 , p45 , p45/SUG

Cellular Localization

Cytoplasm , Nucleus

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-PSMC5 antibody [25D5] used in Western Blot (WB). GTX70288

GTX70288 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSMC5 antibody [25D5] (GTX70288) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PSMC5 antibody [25D5] used in Western Blot (WB). GTX70288

GTX70288 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: Hela
E: Hep G2 (GTX27900)
F: Molt-4 (GTX27912)
G: Raji
10% SDS PAGE
GTX70288 diluted at 1:1000

REFERENCE

Application Reference

Mooser C et al. Nat Commun 2020; 11 (1) : 123 Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations