Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: Hela
E: Hep G2 (GTX27900)
F: Molt-4 (GTX27912)
G: Raji
10% SDS PAGE
GTX70288 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSMC5 antibody [25D5] (GTX70288) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.