GeneTex
United States (US)

PSMC5 antibody

Anti-PSMC5 antibody used in Western Blot (WB). GTX105347
Anti-PSMC5 antibody used in Western Blot (WB). GTX105347
Anti-PSMC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX105347
Anti-PSMC5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105347
Anti-PSMC5 antibody used in Western Blot (WB). GTX105347

Cat No. GTX105347

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

A431 , HepG2 , Neuro2A , GL261 , C8D30 , PC-12

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSMC5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Proteasome 26S Subunit, Atpase 5,S8,Sug-1,Sug1,Tbp10,Trip1,P45,P45/Sug,Psmc5

Cellular Localization

Cytoplasm (Potential) , Nucleus

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PSMC5 antibody used in Western Blot (WB). GTX105347

GTX105347 WB Image

PSMC5 antibody detects PSMC5 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
10 % SDS-PAGE
PSMC5 antibody (GTX105347) dilution: 1:1000

Anti-PSMC5 antibody used in Western Blot (WB). GTX105347

GTX105347 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hep G2 (GTX27900)
10% SDS PAGE
GTX105347 diluted at 1:1000

Anti-PSMC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX105347

GTX105347 IHC-P Image

PSMC5 antibody detects PSMC5 protein at cytoplasm and nucleus in human hepatocellular carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
PSMC5 antibody (GTX105347) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PSMC5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105347

GTX105347 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using PSMC5(GTX105347) antibody at 1:200 dilution.

Anti-PSMC5 antibody used in Western Blot (WB). GTX105347

GTX105347 WB Image

PSMC5 antibody detects PSMC5 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
10 % SDS-PAGE
PSMC5 antibody (GTX105347) dilution: 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169