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PSMC6 antibody

Anti-PSMC6 antibody used in Western Blot (WB). GTX104314
Anti-PSMC6 antibody used in Western Blot (WB). GTX104314
Anti-PSMC6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104314
Anti-PSMC6 antibody used in IHC (Paraffin sections) (IHC-P). GTX104314

Cat. No. GTX104314

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PSMC6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Mouse brain , 293T

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSMC6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951517

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome 26S subunit, ATPase 6 , SUG2 , p42

Cellular Localization

Cytoplasm , Nucleus

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PSMC6 antibody used in Western Blot (WB). GTX104314

GTX104314 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX104314 diluted at 1:1000

Anti-PSMC6 antibody used in Western Blot (WB). GTX104314

GTX104314 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX104314 diluted at 1:500

Anti-PSMC6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104314

GTX104314 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PSMC6(GTX104314) antibody at 1:500 dilution.

Anti-PSMC6 antibody used in IHC (Paraffin sections) (IHC-P). GTX104314

GTX104314 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using PSMC6(GTX104314) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169