Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX114677 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX114677 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PSMC6(GTX114677) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using PSMC6(GTX114677) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min