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PSMD11 antibody [AT1F4]

Anti-PSMD11 antibody [AT1F4] used in Western Blot (WB). GTX50018

Cat No. GTX50018

Host

Mouse

Clonality

Monoclonal

Clone Name

AT1F4

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:250 ~ 1000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human PSMD11 (1-422aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Proteasome 26S Subunit, Non-Atpase 11 , Rpn6 , S9 , P44.5 , Psmd11

Background

PSMD11 (Proteasome 26S non-ATPase subunit 11), also known as S9, Rpn6 or p44.5, has multi-subunit protease complexes consisting of 20S subunits composed of four seven-numbered rings with two outer rings containing alpha subunits and two central rings composed of beta subunits, and 19S caps of 6 ATPase and 11 non-ATPase subunits. PSMD11 is the main proteolytic enzyme that functions in ATP-dependent degradation of ubiquitinated proteins.

Database

Research Area

DATA IMAGES

Anti-PSMD11 antibody [AT1F4] used in Western Blot (WB). GTX50018

GTX50018 WB Image

The extracts of mouse kidney (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PSMD11 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf