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PSMD11 antibody [AT1F4]

Anti-PSMD11 antibody [AT1F4] used in Western Blot (WB). GTX50018
Anti-PSMD11 antibody [AT1F4] used in Western Blot (WB). GTX50018
Anti-PSMD11 antibody [AT1F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50018

Cat. No. GTX50018

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT1F4

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all PSMD11 products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:250 ~ 1000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human PSMD11 (1-422aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

RRID

AB_11172082

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome 26S subunit, non-ATPase 11 , Rpn6 , S9 , p44.5

Cellular Localization

Nucleus , Cytoplasm, cytosol

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S9 family that functions as a non-ATPase subunit of the 19S regulator and is phosphorylated by AMP-activated protein kinase. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES

Anti-PSMD11 antibody [AT1F4] used in Western Blot (WB). GTX50018

GTX50018 WB Image

The extracts of mouse kidney (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PSMD11 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system

Anti-PSMD11 antibody [AT1F4] used in Western Blot (WB). GTX50018

GTX50018 WB Image

The data was published in the journal Sci Rep in 2019. PMID: 31417156

Anti-PSMD11 antibody [AT1F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50018

GTX50018 ICC/IF Image

The data was published in the journal Sci Rep in 2019. PMID: 31417156

REFERENCE

Application Reference

Lee Y et al. Sci Rep 2019; 9 (1) : 11883 The NRON complex controls circadian clock function through regulated PER and CRY nuclear translocation.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399