APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:2,000 |
| 1:250 |
Not tested in other applications.
Calculated MW
Product Note
This antibody detects ~42, 46, 48, and 52 kDa proteins which correspond to subunit 10B and probably the combined signal from 4, 6B, 7, and 8 subunit proteins.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Immunogen
Synthetic Peptide: C A(278) T N R P D T L D P A L L R P G R(294)
Purification
Ammonium sulfate precipitation
Conjugation
Unconjugated
RRID
AB_11174017
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
proteasome 26S subunit, non-ATPase 2 , P97 , RPN1 , S2 , TRAP2
Background
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
Database
Research Area
DATA IMAGES
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GTX79499 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX79499 PSMD2 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature
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GTX79499 WB Image
WB analysis of MCF7 (Lane 1), PC-3 (Lane 2), Hep G2 (Lane 3), SK-OV-3 (Lane 4), HeLa (Lane 5), COS-7 (Lane 6), Jurkat (Lane 7), A-431 (Lane 8), tissue extracts of mouse testis (Lane 9) and rat testis (Lane 10) using GTX79499 PSMD2 antibody.
Dilution : 1:1000
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REFERENCE
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REVIEW
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