APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.93 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PSMD4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10619686
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
proteasome 26S subunit, non-ATPase 4 , AF , AF-1 , ASF , MCB1 , Rpn10 , S5A , pUB-R5
Background
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-PSMD4 antibody [N2C3] used in Western Blot (WB). GTX114679](/templates/default/js/lightbox/images/loading.gif "Anti-PSMD4 antibody [N2C3] used in Western Blot (WB). GTX114679")
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GTX114679 WB Image
Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX114679 diluted at 1:1000
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GTX114679 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PSMD4(GTX114679) antibody at 1:200 dilution.
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REFERENCE
There are currently no references for PSMD4 antibody [N2C3] (GTX114679). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PSMD4 antibody [N2C3] (GTX114679). Be the first to share your experience with this product.
![Anti-PSMD4 antibody [N2C3] used in Western Blot (WB). GTX114679](/upload/website/prouct_img/small/GTX114679/GTX114679_WB_w_23060518_743.webp "Anti-PSMD4 antibody [N2C3] used in Western Blot (WB). GTX114679")
![Anti-PSMD4 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114679](/upload/website/prouct_img/small/GTX114679/GTX114679_IFA_w_23060518_522.webp "Anti-PSMD4 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114679")
![Anti-PSMD4 antibody [N2C3] used in Western Blot (WB). GTX114679](/upload/website/prouct_img/normal/GTX114679/GTX114679_WB_w_23060518_743.webp "Anti-PSMD4 antibody [N2C3] used in Western Blot (WB). GTX114679")
![Anti-PSMD4 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114679](/upload/website/prouct_img/normal/GTX114679/GTX114679_IFA_w_23060518_522.webp "Anti-PSMD4 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114679")