GeneTex
United States (US)

PSMD7 antibody

GTX103677_40527_WB_19011506_370.jpg
GTX103677_40527_IFA_19011506_168.jpg

Cat No. GTX103677

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSMD7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MOV34 antibody, P40 antibody, Rpn8 antibody, S12 antibody, PSMD7 antibody, 26S proteasome regulatory subunit S12 antibody, proteasome subunit p40 antibody, Mov34 homolog antibody, Moloney leukemia virus-34 proviral integration antibody, 26S proteasome non-ATPase regulatory subunit 7 antibody, "proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) antibody", 26S proteasome regulatory subunit rpn8 antibody, "proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 antibody"

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 17. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX103677_40527_WB_19011506_370.jpg

GTX103677 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX103677 diluted at 1:500

GTX103677_40527_IFA_19011506_168.jpg

GTX103677 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PSMD7(GTX103677) antibody at 1:500 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf