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PSME3 antibody [4B2-C2-G4]

Anti-PSME3 antibody [4B2-C2-G4] used in Western Blot (WB). GTX49175

Cat No. GTX49175

Host

Mouse

Clonality

Monoclonal

Clone Name

4B2-C2-G4

Isotype

IgG1

Application

WB

Reactivity

Human
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human PSME3 protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Proteasome Activator Subunit 3 , Hel-S-283 , Ki , Pa28-Gamma , Pa28G , Pa28Gamma , Reg-Gamma , Psme3

Cellular Localization

Nucleus. Cytoplasm By similarity.

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Two transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PSME3 antibody [4B2-C2-G4] used in Western Blot (WB). GTX49175

GTX49175 WB Image

WB analysis of HepG2, Jurkat and K562 lysates using PSME3 antibody at a dilution of 1:1,000.

REFERENCE

There are currently no references for PSME3 antibody [4B2-C2-G4] (GTX49175). Be the first to share your publications with this product.

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf