GeneTex
United States (US)

PSME3 antibody

KO/KD
GTX106722_40135_20180105_WB_KO_watermark_19011506_810.jpg
GTX106722_IHC_19011506_386.jpg
GTX106722_40135_WB_19011506_442.jpg
GTX106722_40135_WB_R_19011506_574.jpg
GTX106722_40135_20150529_IHC_M_19011506_672.jpg
GTX106722_40135_WB_M_19011506_936.jpg
GTX106722_40135_IFA_19011506_468.jpg

Cat No. GTX106722

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

293T , A549 , H1299 , HCT116 , MCF-7 , NIH-3T3 , JC , BCL-1 , PC-12

Predict Reactivity

Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSME3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ki antibody, PA28-gamma antibody, PA28G antibody, REG-GAMMA antibody, PSME3 antibody, Ki nuclear autoantigen antibody, proteasome activator 28 subunit gamma antibody, PA28gamma antibody, proteasome activator 28-gamma antibody, 11S regulator complex subunit gamma antibody, 11S regulator complex gamma subunit antibody, activator of multicatalytic protease subunit 3 antibody, Ki antigen antibody, proteasome activator complex subunit 3 antibody, "proteasome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki) antibody"

Cellular Localization

Nucleus , Cytoplasm (By similarity)

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Two transcript variants encoding different isoforms have been identified. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX106722_40135_20180105_WB_KO_watermark_19011506_810.jpg

GTX106722 WB Image

KO/KD

Wild-type (WT) and PSME3 knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PSME3 antibody (GTX106722) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX106722_IHC_19011506_386.jpg

GTX106722 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using PSME3(GTX106722) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106722_40135_WB_19011506_442.jpg

GTX106722 WB Image

Sample (30 µg of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
12% SDS PAGE
GTX106722 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106722_40135_WB_R_19011506_574.jpg

GTX106722 WB Image

Sample (30 µg of whole cell lysate)
A: PC-12
12% SDS PAGE
GTX106722 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106722_40135_20150529_IHC_M_19011506_672.jpg

GTX106722 IHC-P Image

PSME3 antibody detects PSME3 protein at nucleus in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
PSME3 antibody (GTX106722) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106722_40135_WB_M_19011506_936.jpg

GTX106722 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
12% SDS PAGE
GTX106722 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106722_40135_IFA_19011506_468.jpg

GTX106722 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PSME3(GTX106722) antibody at 1:500 dilution.

Reference
SDS
Glycerol.pdf
Thimerosal.pdf