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United States (US)

PSME3 antibody

Anti-PSME3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32822
Anti-PSME3 antibody used in Western Blot (WB). GTX32822

Cat No. GTX32822

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

30 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-254 of human PSME3 (NP_789839.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Proteasome Activator Subunit 3,Hel-S-283,Ki,Pa28-Gamma,Pa28G,Pa28Gamma,Reg-Gamma,Psme3

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulat

Database

Research Area

DATA IMAGES
Anti-PSME3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32822

GTX32822 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX32822 PSME3 antibody.
Blue : DAPI

Anti-PSME3 antibody used in Western Blot (WB). GTX32822

GTX32822 WB Image

WB analysis of various samples using GTX32822 PSME3 antibody.
Dilution : 1:1000
Loading : 25μg per lane