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PSPH antibody

Anti-PSPH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109163
Anti-PSPH antibody used in Western Blot (WB). GTX109163

Cat. No. GTX109163

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PSPH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human PSPH Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951523

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoserine phosphatase , PSP , PSPHD

Background

The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PSPH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109163

GTX109163 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PSPH(GTX109163) antibody at 1:200 dilution.

Anti-PSPH antibody used in Western Blot (WB). GTX109163

GTX109163 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PSPH antibody (GTX109163) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

O'Leary EM et al. Am J Respir Cell Mol Biol 2020; TGF-β Promotes Metabolic Reprogramming in Lung Fibroblasts via mTORC1-dependent ATF4 Activation.
Application : WB
Reactivity : Human
Selvarajah B et al. Sci Signal 2019; 12 (582) mTORC1 amplifies the ATF4-dependent de novo serine-glycine pathway to supply glycine during TGF-帣1-induced collagen biosynthesis.
Guo H et al. Cancer Lett 2019; 466 : 39-48 NRF2 SUMOylation promotes de novo serine synthesis and maintains HCC tumorigenesis.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169