GeneTex
United States (US)

PSR antibody

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GTX10526_20170605_WB_2_19011506_454.jpg
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Cat No. GTX10526

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500~1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

NIH3T3

Specificity/Sensitivity

Anti-Phosphatidylserine Receptor (PSR) recognizes human and mouse phosphatidylserine receptor (PSR). Detection of the phosphatidylserine receptor by immunoblotting is specifically inhibited by the immunizing peptide, a synthetic peptide corresponding to amino acid residues 363-381 of the human phosphatidylserine receptor, conjugated to maleimide-activated KLH as immunogen.
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 1% bovine serum albumin, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1-1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide corresponding to amino acid residues 363-381 of the human phosphatidylserine receptor conjugated to maleimide-activated KLH. The corresponding sequence in mouse differs by one amino acid.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Jumonji Domain Containing 6, Arginine Demethylase And Lysine Hydroxylase,Psr,Ptdsr,Ptdsr1,Jmjd6

Background

Most cells that undergo apoptosis are rapidly recognized, engulfed, and digested by professional phagocytes or by neighboring cells and eliminated from the organism. In this way, tissue exposure to potentially cytotoxic, immunogenic, or inflammatory cellular contents is prevented. As a result neither inflammation nor inappropriate immune responses are induced in this process. Removal of apoptotic cells comprise the following steps: surface alteration and ligand expression on the apoptotic cell, ligand recognition by tethering receptors on the phagocyte, initiation of appropriate signaling pathways in the phagocyte, engulfment and finally, digestion. Various molecules including proteins, carbohydrates, and phospholipids have been suggested to act as cell surface exposed markers for recognition by phagocytes. Virtually all apoptotic cells permanently express phosphatidylserine (PS) on the outer leaflet of their plasma membrane. Such loss of phospholipids asymmetry and exposure of PS does not occur in resting cells and appears only transiently following activation of certain cells. Phosphatidylserine Receptor (PSR) has been reported and shown to recognize phosphatidylserine in a stereospecific manner. PSR is an evolutionarily conserved glycoprotein that is widely expressed in cells and tissues. It is very susceptible to cleavage by protease or elastase. PSR engagement plays an important role in macropinocytosis and uptake of tethered apoptotic cells. Cooperation with other receptors and their ligands may also be needed for ingestion of apoptotic cells. PSR is also involved in suppression of inflammatory and immune responses.

Database

Research Area

DATA IMAGES
GTX10526_20170605_WB_1_19011506_347.jpg

GTX10526 WB Image

WB analysis of (Lane 1- 2) HeLa, (Lane 3 -4) Jurkat, (Lane 5- 6) Molt-4 cell lysate using GTX10526 PSR antibody.
Lane 1,3,5:. Antibody 1 μg/mL
Lane 2,4,6: Antibody 1 μg/mL + PSR immunizing peptide

GTX10526_20170605_WB_2_19011506_454.jpg

GTX10526 WB Image

WB analysis of K562 cell lysate using GTX10526 PSR antibody.
Lane 1 : Antibody 1 μg/mL
Lane 2 : Antibody 1 μg/mL + PSR immunizing peptide

GTX10526_20170605_WB_19011506_832.jpg

GTX10526 WB Image

WB analysis of A431 cell lysate using GTX10526 PSR antibody.
Lane 1 : Antibody 1 μg/mL
Lane 2 : Antibody 1 μg/mL + PSR immunizing peptide

Package List Price ($)
$ 329