Wild-type (WT) and PSR knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSR antibody (GTX110746) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using PSR(GTX110746) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX110746 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX110746 diluted at 1:1000