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PSR antibody

KO/KD
Anti-PSR antibody used in Western Blot (WB). GTX110746
Anti-PSR antibody used in Western Blot (WB). GTX110746
Anti-PSR antibody used in IHC (Paraffin sections) (IHC-P). GTX110746
Anti-PSR antibody used in Western Blot (WB). GTX110746

Cat. No. GTX110746

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PSR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

293T , NIH-3T3

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950607

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

jumonji domain containing 6, arginine demethylase and lysine hydroxylase , PSR , PTDSR , PTDSR1

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have indicated that it does not directly function in the clearance of apoptotic cells, and questioned whether it is a true phosphatidylserine receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PSR antibody used in Western Blot (WB). GTX110746

GTX110746 WB Image

KO/KD

Wild-type (WT) and PSR knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSR antibody (GTX110746) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PSR antibody used in Western Blot (WB). GTX110746

GTX110746 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX110746 diluted at 1:1000

Anti-PSR antibody used in IHC (Paraffin sections) (IHC-P). GTX110746

GTX110746 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using PSR(GTX110746) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PSR antibody used in Western Blot (WB). GTX110746

GTX110746 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX110746 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169