APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.01-0.3μg/ml |
| 2-4μg/ml |
|
Note :
IHC-P
Human Kidney shows nuclear membrane staining in all the convoluted tubules.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX25642-PEP).
Product Note
This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).
Predict Reactivity
Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence DGIVPDIAVGTKR-C, from the N Terminus of the protein sequence according to NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
polypyrimidine tract binding protein 1 , HNRNP-I , HNRNPI , HNRPI , PTB , PTB-1 , PTB-T , PTB2 , PTB3 , PTB4 , pPTB
Cellular Localization
Nucleus
Background
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX25642 WB Image
WB analysis of mouse ovary (A) and rat ovary (B) lysate using GTX25642 PTBP1 antibody, N-term.
Dilution : 0.1-0.3μg/ml
Loading : 35μg protein in RIPA buffer
|
|
|
GTX25642 IHC-P Image
IHC-P analysis of human kidney using GTX25642 PTBP1 antibody, N-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 2μg/ml
|
|
|
GTX25642 WB Image
WB analysis of human ovary lysate using GTX25642 PTBP1 antibody, N-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
|
|
|
GTX25642 WB Image
WB analysis of NIH-3T3 cell lysate using GTX25642 PTBP1 antibody, N-term.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer
|
|
|
GTX25642 WB Image
WB analysis of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates using GTX25642 PTBP1 antibody, N-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for PTBP1 antibody, N-term (GTX25642). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PTBP1 antibody, N-term (GTX25642). Be the first to share your experience with this product.
