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PTBP2 antibody [C1C3]

Anti-PTBP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX116703
Anti-PTBP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX116703
Anti-PTBP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX116703
Anti-PTBP2 antibody [C1C3] used in Western Blot (WB). GTX116703
Anti-PTBP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX116703
Anti-PTBP2 antibody [C1C3] used in Western Blot (WB). GTX116703

Cat. No. GTX116703

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PTBP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116

Predict Reactivity

Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PTBP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615657

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polypyrimidine tract binding protein 2 , PTBLP , brPTB , nPTB

Background

The protein encoded by this gene binds to the intronic cluster of RNA regulatory elements, downstream control sequence (DCS). It is implicated in controlling the assembly of other splicing-regulatory proteins. This protein is very similar to the polypyrimidine tract binding protein but it is expressed primarily in the brain. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PTBP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX116703

GTX116703 IHC-P Image

PTBP2 antibody [C1C3] detects PTBP2 protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
PTBP2 antibody [C1C3] (GTX116703) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PTBP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX116703

GTX116703 IHC-P Image

PTBP2 antibody [C1C3] detects PTBP2 protein at nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
PTBP2 antibody [C1C3] (GTX116703) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PTBP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX116703

GTX116703 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PTBP2(GTX116703) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PTBP2 antibody [C1C3] used in Western Blot (WB). GTX116703

GTX116703 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX116703 diluted at 1:1000

Anti-PTBP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX116703

GTX116703 IHC-P Image

PTBP2 antibody [C1C3] detects PTBP2 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PTBP2 antibody [C1C3] (GTX116703) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PTBP2 antibody [C1C3] used in Western Blot (WB). GTX116703

GTX116703 WB Image

PTBP2 antibody [C1C3] detects PTBP2 protein by western blot analysis.
A. 30 μg H1299 whole cell lysate/extract
B. 30 μg HCT116 whole cell lysate/extract
7.5 % SDS-PAGE
PTBP2 antibody [C1C3] (GTX116703) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169