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PTBP2 antibody

Anti-PTBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116704
Anti-PTBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116704
Anti-PTBP2 antibody used in Western Blot (WB). GTX116704
Anti-PTBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116704
Anti-PTBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116704
Anti-PTBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116704
Anti-PTBP2 antibody used in Western Blot (WB). GTX116704

Cat. No. GTX116704

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PTBP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , Mouse eye

Predict Reactivity

Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PTBP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885401

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polypyrimidine tract binding protein 2 , PTBLP , brPTB , nPTB

Cellular Localization

Nucleus

Background

The protein encoded by this gene binds to the intronic cluster of RNA regulatory elements, downstream control sequence (DCS). It is implicated in controlling the assembly of other splicing-regulatory proteins. This protein is very similar to the polypyrimidine tract binding protein but it is expressed primarily in the brain. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PTBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116704

GTX116704 ICC/IF Image

PTBP2 antibody detects PTBP2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PTBP2 stained by PTBP2 antibody (GTX116704) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PTBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116704

GTX116704 ICC/IF Image

PTBP2 antibody detects polypyrimidine tract binding protein 2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: polypyrimidine tract binding protein 2 protein stained by PTBP2 antibody (GTX116704) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PTBP2 antibody used in Western Blot (WB). GTX116704

GTX116704 WB Image

PTBP2 antibody detects PTBP2 protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HCT116 whole cell lysate/extract
7.5 % SDS-PAGE
PTBP2 antibody (GTX116704) dilution: 1:2000

Anti-PTBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116704

GTX116704 IHC-P Image

PTBP2 antibody detects PTBP2 protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
PTBP2 antibody (GTX116704) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PTBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116704

GTX116704 IHC-P Image

PTBP2 antibody detects PTBP2 protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
PTBP2 antibody (GTX116704) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PTBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116704

GTX116704 IHC-P Image

PTBP2 antibody detects PTBP2 protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
PTBP2 antibody (GTX116704) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PTBP2 antibody used in Western Blot (WB). GTX116704

GTX116704 WB Image

PTBP2 antibody detects PTBP2 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PTBP2 antibody (GTX116704) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169