APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 5μg/ml |
|
Note :
ICC/IF
Expression of the protein seen in the Golgi/membrane of NIH3T3 cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89309-PEP).
Product Note
This antibody is expected to recognise all four reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1).
Predict Reactivity
Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-HPESRHHPPSNPRQQ, from the internal region of the protein sequence according to NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
patched 1 , BCNS , NBCCS , PTC , PTC1 , PTCH
Cellular Localization
Cell membrane
Background
This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
|
GTX89309 ICC/IF Image
ICC/IF analysis of PFA fixed NIH-3T3 cells using GTX89309 PTCH1 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 5μg/ml
|
|
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GTX89309 WB Image
WB analysis of human brain lysate using GTX89309 PTCH1 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for PTCH1 antibody, Internal (GTX89309). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PTCH1 antibody, Internal (GTX89309). Be the first to share your experience with this product.
