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PTEN antibody [1B8]

Anti-PTEN antibody [1B8] used in Flow cytometry (FACS). GTX83304
Anti-PTEN antibody [1B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83304
Anti-PTEN antibody [1B8] used in Western Blot (WB). GTX83304

Cat. No. GTX83304

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA, PLA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all PTEN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P Assay dependent
FACS 1/200 - 1/400
ELISA 1/10000
PLA Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of PTEN expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11174201

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatase and tensin homolog , 10q23del , BZS , CWS1 , DEC , GLM2 , MHAM , MMAC1 , PTEN1 , PTENbeta , TEP1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PTEN antibody [1B8] used in Flow cytometry (FACS). GTX83304

GTX83304 FACS Image

FACS analysis of HeLa cells using GTX83304 PTEN antibody [1B8].
Right : PTEN
Left : negative control

Anti-PTEN antibody [1B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83304

GTX83304 ICC/IF Image

ICC/IF analysis of HeLa (left) and HepG2 (right) cells using GTX83304 PTEN antibody [1B8].
Green : PTEN
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-PTEN antibody [1B8] used in Western Blot (WB). GTX83304

GTX83304 WB Image

WB analysis of HeLa (1) and NIH3T3 (2) cell lysate using GTX83304 PTEN antibody [1B8].

REFERENCE

Application Reference

Cizkova K et al. Biology (Basel) 2022; 11 (7) Lipid Messenger Phosphatidylinositol-4,5-Bisphosphate Is Increased by Both PPARα Activators and Inhibitors: Relevance for Intestinal Cell Differentiation.
Application : ICC/IF, IHC-P, ELISA, PLA
Reactivity : Human
Ma JJ et al. J Cell Physiol 2019; (Epub) Regulation of adult mammalian intrinsic axonal regeneration by NF-庥B/STAT3 signaling cascade.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399