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PTEN antibody [9E8]

Anti-PTEN antibody [9E8] used in IHC (Paraffin sections) (IHC-P). GTX34151

Cat No. GTX34151

Host

Mouse

Clonality

Monoclonal

Clone Name

9E8

Isotype

IgG1

Application

IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1:100-1:200
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide derived from PTEN at 320-400 aa.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BZS,CWS1,DEC,GLM2,MHAM,MMAC1,PTEN,PTEN1,TEP1,phosphatase and tensin homolog,10q23del

Cellular Localization

Cytoplasm,Nucleus,PML body,Secreted

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]

Database

Research Area

DATA IMAGES

Anti-PTEN antibody [9E8] used in IHC (Paraffin sections) (IHC-P). GTX34151

GTX34151 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX34151 PTEN antibody [9E8].
Dilution : 1:200

REFERENCE

There are currently no references for PTEN antibody [9E8] (GTX34151). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PTEN antibody [9E8] (GTX34151). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319