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PTEN antibody [PTEN/2110]

Anti-PTEN antibody [PTEN/2110] used in IHC (Paraffin sections) (IHC-P). GTX17980
Anti-PTEN antibody [PTEN/2110] used in Protein Array (Protein Array). GTX17980
Anti-PTEN antibody [PTEN/2110] used in IHC (Paraffin sections) (IHC-P). GTX17980

Cat. No. GTX17980

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 PTEN/2110

Isotype

 IgG2b

Application

 IHC-P, Protein Array

Reactivity

 Human
Package
100 μg ($529)
See all PTEN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1-2μg/ml for 30 minutes at RT
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human PTEN protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BZS , CWS1 , DEC , GLM2 , MHAM , MMAC1 , PTEN , PTEN1 , TEP1 , phosphatase and tensin homolog , 10q23del

Cellular Localization

Cytoplasm,Nucleus,PML body,Secreted

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]

Database

Research Area

DATA IMAGES

Anti-PTEN antibody [PTEN/2110] used in IHC (Paraffin sections) (IHC-P). GTX17980

GTX17980 IHC-P Image

IHC-P analysis of human renal cell carcinoma tissue using GTX17980 PTEN antibody [PTEN/2110].

Anti-PTEN antibody [PTEN/2110] used in Protein Array (Protein Array). GTX17980

GTX17980 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using PTEN Mouse Monoclonal Antibody (PTEN/2110). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-PTEN antibody [PTEN/2110] used in IHC (Paraffin sections) (IHC-P). GTX17980

GTX17980 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX17980 PTEN antibody [PTEN/2110].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529