PRODUCT
Summary
PTEN antibody detects phosphatase and tensin homolog (PTEN), a known tumor suppressor protein with a predicted molecular weight of ~47 kDa. PTEN is a key phosphatase involved in the AKT pathway, playing important roles in cell growth, survival, and migration. Loss of PTEN function is found in various diseases, including cancers.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , 293T , PC-12
Predict Reactivity
Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.68 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PTEN. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1241223
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phosphatase and tensin homolog , 10q23del , BZS , CWS1 , DEC , GLM2 , MHAM , MMAC1 , PTEN1 , PTENbeta , TEP1
Cellular Localization
Cytoplasm
Background
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101025 WB Image
Wild-type (WT) and PTEN knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101025 WB Image
Sample (30 μg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX101025 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101025 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101025 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PTEN(GTX101025) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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GTX101025 IHC-P Image
PTEN antibody detects PTEN protein at cytoplasm in human ovarian cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
PTEN antibody (GTX101025) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101025 IHC-P Image
PTEN antibody detects PTEN protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).Sample: Paraffin-embedded human normal colon tissue.PTEN stained by PTEN antibody (GTX101025) diluted at 1:500.Antigen Retrieval: Citrate buffer, 20 min
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GTX101025 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101025 IHC-P Image
PTEN antibody detects PTEN protein at cytosol and nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
PTEN antibody (GTX101025) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101025 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101025 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101025 IHC-P Image
PTEN antibody detects PTEN protein at cytosol and nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
PTEN antibody (GTX101025) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101025 WB Image
The data was published in the journal Sci Rep in 2016. PMID: 26838546
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GTX101025 ICC/IF Image
The data was published in the journal Int J Mol Sci in 2017. PMID: 29207527
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GTX101025 ICC/IF Image
The data was published in the journal Int J Mol Sci in 2017. PMID: 29207527
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REFERENCE
REVIEW
PTEN antibody
Cat. No. GTX101025
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Rating
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( Average 4 based on 2 users reviews)
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Application
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Western Blot(WB)
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( Average 3 based on 1 users reviews)
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 21-Oct-2020
Application Tested :
IHC-P
Sample Species :
Hu
Sample :
normal colon tissue
Fixation :
Citrate buffer, 0.3°C
Primary Antibody :
1:500, 4°C, 16Hr
Date :
Anonymous submitted on 26-Apr-2012
Application Tested :
WB
Sample Species :
Hu
Sample :
Human embryonic stem cells
Amount used :
30μg
Blocking :
5% milk in PBST, 25°C, 0.5Hr
Primary Antibody :
1:1000, 4°C, 16Hr
