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PTEN antibody

Anti-PTEN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101025
Anti-PTEN antibody used in Western Blot (WB). GTX101025
Anti-PTEN antibody used in IHC (Paraffin sections) (IHC-P). GTX101025
Anti-PTEN antibody used in IHC (Paraffin sections) (IHC-P). GTX101025
Anti-PTEN antibody used in Western Blot (WB). GTX101025
Anti-PTEN antibody used in Western Blot (WB). GTX101025
Anti-PTEN antibody used in IHC (Paraffin sections) (IHC-P). GTX101025
Anti-PTEN antibody used in Western Blot (WB). GTX101025
Anti-PTEN antibody used in Western Blot (WB). GTX101025

Cat No. GTX101025

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T, A549 , H1299 , HCT116 , MCF-7 , BCL-1 , NIH-3T3 , PC-12 , *Human embryonic stem cell

Predict Reactivity

Bovine, Dog, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PTEN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

phosphatase and tensin homolog , 10q23del , BZS , CWS1 , DEC , GLM2 , MHAM , MMAC1 , PTEN1 , PTENbeta , TEP1

Cellular Localization

Cytoplasm

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PTEN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101025

GTX101025 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PTEN(GTX101025) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-PTEN antibody used in Western Blot (WB). GTX101025

GTX101025 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX101025 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PTEN antibody used in IHC (Paraffin sections) (IHC-P). GTX101025

GTX101025 IHC-P Image

PTEN antibody detects PTEN protein at cytosol and nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
PTEN antibody (GTX101025) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PTEN antibody used in IHC (Paraffin sections) (IHC-P). GTX101025

GTX101025 IHC-P Image

PTEN antibody detects PTEN protein at cytosol and nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
PTEN antibody (GTX101025) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PTEN antibody used in Western Blot (WB). GTX101025

GTX101025 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PTEN antibody used in Western Blot (WB). GTX101025

GTX101025 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PTEN antibody used in IHC (Paraffin sections) (IHC-P). GTX101025

GTX101025 IHC-P Image

PTEN antibody detects PTEN protein at cytoplasm in human ovarian cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
PTEN antibody (GTX101025) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PTEN antibody used in Western Blot (WB). GTX101025

GTX101025 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PTEN antibody used in Western Blot (WB). GTX101025

GTX101025 WB Image

Wild-type (WT) and PTEN knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

PTEN antibody Cat No. GTX101025

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 26-Apr-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human embryonic stem cells
Amount used : 30μg
Blocking : 5% milk in PBST, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169