GeneTex
United States (US)

PTEN antibody

GTX101025_40793_20150515_WB_Merged_S_19011506_214.jpg
GTX101025_40324_IFA_19011506_969.jpg
GTX101025_39694_WB_M_19011506_648.jpg
GTX101025_39693_IHC_M_19011506_947.jpg
GTX101025_39693_IHC_R_19011506_578.jpg
GTX101025_40793_20160204_WB_R_19011506_992.jpg
GTX101025_40793_20160128_WB_19011506_959.jpg
GTX101025_40793_20160125_IHC-P_19011506_896.jpg

Cat No. GTX101025

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T, A549 , H1299 , HCT116 , MCF-7 , BCL-1 , NIH-3T3 , PC-12 , *Human embryonic stem cell

Predict Reactivity

Bovine, Dog, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PTEN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphatase And Tensin Homolog,10Q23Del,Bzs,Cws1,Dec,Glm2,Mham,Mmac1,Pten1,Ptenbeta,Tep1,Pten

Cellular Localization

Cytoplasm

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101025_40793_20150515_WB_Merged_S_19011506_214.jpg

GTX101025 WB Image

PTEN antibody detects PTEN protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101025_40324_IFA_19011506_969.jpg

GTX101025 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PTEN(GTX101025) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101025_39694_WB_M_19011506_648.jpg

GTX101025 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX101025 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101025_39693_IHC_M_19011506_947.jpg

GTX101025 IHC-P Image

PTEN antibody detects PTEN protein at cytosol and nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
PTEN antibody (GTX101025) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101025_39693_IHC_R_19011506_578.jpg

GTX101025 IHC-P Image

PTEN antibody detects PTEN protein at cytosol and nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
PTEN antibody (GTX101025) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101025_40793_20160204_WB_R_19011506_992.jpg

GTX101025 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101025_40793_20160128_WB_19011506_959.jpg

GTX101025 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PTEN antibody (GTX101025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101025_40793_20160125_IHC-P_19011506_896.jpg

GTX101025 IHC-P Image

PTEN antibody detects PTEN protein at cytoplasm in human ovarian cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
PTEN antibody (GTX101025) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

PTEN antibody Cat No. GTX101025

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 26-Apr-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human embryonic stem cells
Amount used : 30μg
Blocking : 5% milk in PBST, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169
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