WB (peptide competition) analysis of 3T3-L1 cells using GTX60929 PTEN (phospho Ser370) antibody prior incubated with non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 1), a generic phosphoserine-containing peptide (Lane 2), the phosphopeptide immunogen (Lane 3) or no peptide (Lane 4, 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

IHC-P analysis of human breast carcinoma tissue using GTX60929 PTEN (phospho Ser370) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

ICC/IF analysis of SH-SY5Y cells using GTX60929 PTEN (phospho Ser370) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature

IHC-P analysis of mouse prostate tissue using GTX60929 PTEN (phospho Ser370) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

IHC-P analysis of human prostate carcinoma tissue using GTX60929 PTEN (phospho Ser370) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

WB analysis of 20 μg of MCF-7 (Lane 1), HeLa (Lane 2), NIH/3T3 (Lane 3), Mouse Brain (Lane 4), Rat Brain (Lane 5) and SHSY5Y (Lane 6) cell lysate using GTX60929 PTEN (phospho Ser370) antibody.
Dilution : 1:1000