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PTEN (phospho Ser370) antibody

Orthogonal
Anti-PTEN (phospho Ser370) antibody used in Western Blot (WB). GTX60929
Anti-PTEN (phospho Ser370) antibody used in IHC (Paraffin sections) (IHC-P). GTX60929
Anti-PTEN (phospho Ser370) antibody used in IHC (Paraffin sections) (IHC-P). GTX60929
Anti-PTEN (phospho Ser370) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60929
Anti-PTEN (phospho Ser370) antibody used in IHC (Paraffin sections) (IHC-P). GTX60929
Anti-PTEN (phospho Ser370) antibody used in Western Blot (WB). GTX60929

Cat. No. GTX60929

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
50 μl ($399)
See all PTEN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:250
IHC-P 1:10-1:100
FACS 1:20
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PTEN that contains serine 370. The sequence is conserved in mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatase and tensin homolog , 10q23del , BZS , CWS1 , DEC , GLM2 , MHAM , MMAC1 , PTEN1 , PTENbeta , TEP1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]

Database

Research Area

DATA IMAGES

Anti-PTEN (phospho Ser370) antibody used in Western Blot (WB). GTX60929

GTX60929 WB Image

WB analysis of 20 μg of MCF-7 (Lane 1), HeLa (Lane 2), NIH/3T3 (Lane 3), Mouse Brain (Lane 4), Rat Brain (Lane 5) and SHSY5Y (Lane 6) cell lysate using GTX60929 PTEN (phospho Ser370) antibody.
Dilution : 1:1000

Anti-PTEN (phospho Ser370) antibody used in IHC (Paraffin sections) (IHC-P). GTX60929

GTX60929 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX60929 PTEN (phospho Ser370) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-PTEN (phospho Ser370) antibody used in IHC (Paraffin sections) (IHC-P). GTX60929

GTX60929 IHC-P Image

IHC-P analysis of mouse prostate tissue using GTX60929 PTEN (phospho Ser370) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-PTEN (phospho Ser370) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60929

GTX60929 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX60929 PTEN (phospho Ser370) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature

Anti-PTEN (phospho Ser370) antibody used in IHC (Paraffin sections) (IHC-P). GTX60929

GTX60929 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX60929 PTEN (phospho Ser370) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-PTEN (phospho Ser370) antibody used in Western Blot (WB). GTX60929

GTX60929 WB Image

Orthogonal

WB (peptide competition) analysis of 3T3-L1 cells using GTX60929 PTEN (phospho Ser370) antibody prior incubated with non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 1), a generic phosphoserine-containing peptide (Lane 2), the phosphopeptide immunogen (Lane 3) or no peptide (Lane 4, 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399