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PTEN (phospho Ser380/Thr382/Thr383) antibody

Anti-PTEN (phospho Ser380/Thr382/Thr383) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50130
Anti-PTEN (phospho Ser380/Thr382/Thr383) antibody used in Western Blot (WB). GTX50130
Anti-PTEN (phospho Ser380/Thr382/Thr383) antibody used in Western Blot (WB). GTX50130
Anti-PTEN (phospho Ser380/Thr382/Thr383) antibody used in IHC (Paraffin sections) (IHC-P). GTX50130
Anti-PTEN (phospho Ser380/Thr382/Thr383) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50130

Cat No. GTX50130

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 380/382/383 (R-Y-S(p)-D-T(p)-T(p)-D-S) derived from human PTEN.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphatase and tensin homolog , 10q23del , BZS , CWS1 , DEC , GLM2 , MHAM , MMAC1 , PTEN1 , PTENbeta , TEP1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]

Database

Research Area

DATA IMAGES

Anti-PTEN (phospho Ser380/Thr382/Thr383) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50130

GTX50130 ICC/IF Image

ICC/IF analysis of methanol-fixed MEFcells using GTX50130 PTEN (phospho Ser380/Thr382/Thr383) antibody.

Anti-PTEN (phospho Ser380/Thr382/Thr383) antibody used in Western Blot (WB). GTX50130

GTX50130 WB Image

WB analysis of extracts from HeLa and C6 cellstreated with EGF or PMA using GTX50130 PTEN (phospho Ser380/Thr382/Thr383) antibody.

Anti-PTEN (phospho Ser380/Thr382/Thr383) antibody used in Western Blot (WB). GTX50130

GTX50130 WB Image

WB analysis of extracts from HeLa cells treated with IFNa or calf intestinal phosphatase (CIP) using GTX50130 PTEN (phospho Ser380/Thr382/Thr383) antibody.

Anti-PTEN (phospho Ser380/Thr382/Thr383) antibody used in IHC (Paraffin sections) (IHC-P). GTX50130

GTX50130 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50130 PTEN (phospho Ser380/Thr382/Thr383) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-PTEN (phospho Ser380/Thr382/Thr383) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50130

GTX50130 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50130 PTEN (phospho Ser380/Thr382/Thr383) antibody.

REFERENCE

There are currently no references for PTEN (phospho Ser380/Thr382/Thr383) antibody (GTX50130). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf