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PTEN (phospho Ser380) antibody

Anti-PTEN (phospho Ser380) antibody used in Western Blot (WB). GTX133192
Anti-PTEN (phospho Ser380) antibody used in Western Blot (WB). GTX133192

Cat No. GTX133192

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

NIH3T3 , PC-12 , Rat2

Predict Reactivity

Human, Bovine, Dog, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human PTEN (phospho Ser380). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

phosphatase and tensin homolog,10q23del,BZS,CWS1,DEC,GLM2,MHAM,MMAC1,PTEN1,PTENbeta,TEP1

Cellular Localization

Cytoplasm

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PTEN (phospho Ser380) antibody used in Western Blot (WB). GTX133192

GTX133192 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Ser380) antibody (GTX133192) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PTEN (phospho Ser380) antibody used in Western Blot (WB). GTX133192

GTX133192 WB Image

Untreated (–) and treated (+) NIH3T3 whole cell extracts (60 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Ser380) antibody (GTX133192) diluted at 1:1000.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169