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PTGR1 antibody [N1C2]

Anti-PTGR1 antibody [N1C2] used in Western Blot (WB). GTX118527
Anti-PTGR1 antibody [N1C2] used in Western Blot (WB). GTX118527

Cat. No. GTX118527

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

PL45 , PANC1 , MIAPACA2 , BxPC3 , Capan2

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PTGR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618960

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prostaglandin reductase 1 , LTB4DH , PGR1 , ZADH3

Background

This gene encodes an enzyme that is involved in the inactivation of the chemotactic factor, leukotriene B4. The encoded protein specifically catalyzes the NADP+ dependent conversion of leukotriene B4 to 12-oxo-leukotriene B4. A pseudogene of this gene is found on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-PTGR1 antibody [N1C2] used in Western Blot (WB). GTX118527

GTX118527 WB Image

Wild-type (WT) and PTGR1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PTGR1 antibody [N1C2] (GTX118527) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PTGR1 antibody [N1C2] used in Western Blot (WB). GTX118527

GTX118527 WB Image

Prostaglandin reductase 1 antibody [N1C2] detects Prostaglandin reductase 1 protein by Western blot analysis.
A. 40 μg PL45 whole cell lysate/extract
B. 40 μg PANC1 whole cell lysate/extract
C. 40 μg MIAPACA2 whole cell lysate/extract
D. 40 μg BxPC3 whole cell lysate/extract
E. 40 μg Capan2 whole cell lysate/extract
Prostaglandin reductase 1 antibody [N1C2] (GTX118527) dilution: 1:500

REFERENCE

REVIEW

PTGR1 antibody [N1C2] Cat. No. GTX118527

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 30-May-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : PL45 cells,PANC1 cells,MIAPACA2 cells,BxPC3 cells,Capan2 cells
Amount used : 40μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169