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PTH1R antibody [HL2817]

Recombinant antibody Orthogonal Overexpression
Anti-PTH1R antibody [HL2817] used in Western Blot (WB). GTX639958
Anti-PTH1R antibody [HL2817] used in Western Blot (WB). GTX639958

Cat. No. GTX639958

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2817

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PTH1R products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human PTH1R.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

parathyroid hormone 1 receptor , EKNS , PFE , PTHR , PTHR1

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansen's metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), as well as enchodromatosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-PTH1R antibody [HL2817] used in Western Blot (WB). GTX639958

GTX639958 WB Image

Orthogonal Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PTH1R antibody [HL2817] (GTX639958) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PTH1R antibody [HL2817] used in Western Blot (WB). GTX639958

GTX639958 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PTH1R antibody [HL2817] (GTX639958) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
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Custom Conjugations