PTH1R antibody [HL3651]

PTH1R antibody [HL3651] (GTX641642) detects PTH1R protein by flow cytometry analysis. Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer. The cells were following stained with PTH1R antibody [HL3651] (GTX641642) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) at 4ºC for 1 hour.

PTH1R antibody [HL3651] detects PTH1R protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse kidney.
Green: PTH1R stained by PTH1R antibody [HL3651] (GTX641642) diluted at 1:250.
Red: Vimentin antibody [GT812] (GTX629744) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Unboiled mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PTH1R antibody [HL3651] (GTX641642) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PTH1R antibody [HL3651] (GTX641642) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.