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PTHLH antibody [N1C3]

Anti-PTHLH antibody [N1C3] used in Western Blot (WB). GTX100762
Anti-PTHLH antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100762
Anti-PTHLH antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX100762

Cat No. GTX100762

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

A549 , HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PTHLH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

parathyroid hormone like hormone,BDE2,HHM,PLP,PTHR,PTHRP

Cellular Localization

Cytoplasm , Nucleus , Secreted

Background

The protein encoded by this gene is a member of the parathyroid hormone family. This hormone regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. This hormone is involved in lactation possibly by regulating the mobilization and transfer of calcium to the milk. The receptor of this hormone, PTHR1, is responsible for most cases of humoral hypercalcemia of malignancy. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed. There is also evidence for alternative translation initiation from non-AUG (CUG and GUG) start sites, in-frame and downstream of the initiator AUG codon, to give rise to nuclear forms of this hormone. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PTHLH antibody [N1C3] used in Western Blot (WB). GTX100762

GTX100762 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
12% SDS PAGE
GTX100762 diluted at 1:1000

Anti-PTHLH antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100762

GTX100762 ICC/IF Image

PTHLH antibody [N1C3] detects PTHLH protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PTHLH protein stained by PTHLH antibody [N1C3] (GTX100762) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PTHLH antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX100762

GTX100762 IHC-P Image

PTHLH antibody [N1C3] detects PTHLH protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
PTHLH antibody [N1C3] (GTX100762) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169