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PTK9 antibody

Anti-PTK9 antibody used in IHC (Paraffin sections) (IHC-P). GTX111439
Anti-PTK9 antibody used in Western Blot (WB). GTX111439
Anti-PTK9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111439
Anti-PTK9 antibody used in Western Blot (WB). GTX111439

Cat. No. GTX111439

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PTK9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

NIH-3T3 , H1299

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PTK9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952450

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

twinfilin actin binding protein 1 , A6 , PTK9

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PTK9 antibody used in IHC (Paraffin sections) (IHC-P). GTX111439

GTX111439 IHC-P Image

PTK9 antibody detects PTK9 protein at cytoplasm on SW480 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SW480 xenograft.
PTK9 antibody (GTX111439) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PTK9 antibody used in Western Blot (WB). GTX111439

GTX111439 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
10% SDS PAGE
GTX111439 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PTK9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111439

GTX111439 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PTK9(GTX111439) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-PTK9 antibody used in Western Blot (WB). GTX111439

GTX111439 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX111439 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Samaeekia R et al. Clin Cancer Res 2016; 23 (4) : 1091-1103 MicroRNA-206 inhibits stemness and metastasis of breast cancer by targeting MKL1/IL11 pathway.
Application : WB
Reactivity : Human
Bockhorn J et al. Nat Commun 2013; 4 : 1393 MicroRNA-30c inhibits human breast tumour chemotherapy resistance by regulating TWF1 and IL-11.
Application : WB, IHC
Reactivity : Human
Bockhorn J et al. Breast Cancer Res Treat 2013; 137 (2) : 373-82 MicroRNA-30c targets cytoskeleton genes involved in breast cancer cell invasion.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169