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PTK9 antibody

Anti-PTK9 antibody used in Western Blot (WB). GTX111439
Anti-PTK9 antibody used in Western Blot (WB). GTX111439
Anti-PTK9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111439
Anti-PTK9 antibody used in IHC (Paraffin sections) (IHC-P). GTX111439

Cat. No. GTX111439

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PTK9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

NIH-3T3 , H1299

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PTK9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952450

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

twinfilin actin binding protein 1 , A6 , PTK9

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PTK9 antibody used in Western Blot (WB). GTX111439

GTX111439 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX111439 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PTK9 antibody used in Western Blot (WB). GTX111439

GTX111439 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
10% SDS PAGE
GTX111439 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PTK9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111439

GTX111439 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PTK9(GTX111439) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-PTK9 antibody used in IHC (Paraffin sections) (IHC-P). GTX111439

GTX111439 IHC-P Image

PTK9 antibody detects PTK9 protein at cytoplasm on SW480 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SW480 xenograft.
PTK9 antibody (GTX111439) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Samaeekia R et al. Clin Cancer Res 2016; 23 (4) : 1091-1103 MicroRNA-206 inhibits stemness and metastasis of breast cancer by targeting MKL1/IL11 pathway.
Application : WB
Reactivity : Human
Bockhorn J et al. Nat Commun 2013; 4 : 1393 MicroRNA-30c inhibits human breast tumour chemotherapy resistance by regulating TWF1 and IL-11.
Application : WB, IHC
Reactivity : Human
Bockhorn J et al. Breast Cancer Res Treat 2013; 137 (2) : 373-82 MicroRNA-30c targets cytoskeleton genes involved in breast cancer cell invasion.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169