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PTP1B antibody [4F8F11]

Anti-PTP1B antibody [4F8F11] used in Flow cytometry (FACS). GTX04099
Anti-PTP1B antibody [4F8F11] used in IHC (Paraffin sections) (IHC-P). GTX04099
Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099
Anti-PTP1B antibody [4F8F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04099
Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099
Anti-PTP1B antibody [4F8F11] used in ELISA (ELISA). GTX04099
Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099

Cat. No. GTX04099

Host

Mouse

Clonality

Monoclonal

Clone Name

4F8F11

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:200-1:1000
IHC-P 1:200-1:1000
FACS 1:200-1:400
ELISA 1:10000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PTPN1 (AA: 40-246) expressed in E. Coli.

Purification

Purified IgG1

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein tyrosine phosphatase non-receptor type 1 , PTP1B

Cellular Localization

Endoplasmic reticulum membrane

Background

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-PTP1B antibody [4F8F11] used in Flow cytometry (FACS). GTX04099

GTX04099 FACS Image

FACS analysis of Jurkat cells using GTX04099 PTP1B antibody [4F8F11].
Green : Primary antibody
Red : Negative control

Anti-PTP1B antibody [4F8F11] used in IHC (Paraffin sections) (IHC-P). GTX04099

GTX04099 IHC-P Image

IHC-P analysis of human bladder cancer tissues using GTX04099 PTP1B antibody [4F8F11].

Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099

GTX04099 WB Image

WB analysis of PTP1B (AA: 40-246), human IgG Fc tag transfected HEK293 lysates using GTX04099 PTP1B antibody [4F8F11].
Lane 1 : HEK293
Lane 2 : PTP1B (AA: 40-246), human IgG Fc tag transfected HEK293

Anti-PTP1B antibody [4F8F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04099

GTX04099 ICC/IF Image

ICC/IF analysis of Hela cells using GTX04099 PTP1B antibody [4F8F11].
Green : Primary antibody
Blue : DRAQ5 fluorescent DNA dye
Red : Actin filament

Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099

GTX04099 WB Image

WB analysis of human PTP1B (AA: 40-246) recombinant protein using GTX04099 PTP1B antibody [4F8F11].

Anti-PTP1B antibody [4F8F11] used in ELISA (ELISA). GTX04099

GTX04099 ELISA Image

ELISA analysis of antigen proteins using GTX04099 PTP1B antibody [4F8F11].
Black line : Control Antigen (100 ng)
Purple line : Antigen (10 ng)
Blue line : Antigen (50 ng)
Red line : Antigen (100 ng)

Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099

GTX04099 WB Image

WB analysis of various cell lysates using GTX04099 PTP1B antibody [4F8F11].
Lane 1 : Jurkat cell lysates
Lane 2 : MCF-7 cell lysates

REFERENCE

There are currently no references for PTP1B antibody [4F8F11] (GTX04099). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PTP1B antibody [4F8F11] (GTX04099). Be the first to share your experience with this product.
Package List Price ($)
$ 399