APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:200-1:1000 |
| 1:200-1:1000 |
| 1:200-1:400 |
| 1:10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human PTPN1 (AA: 40-246) expressed in E. Coli.
Purification
Purified IgG1
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein tyrosine phosphatase non-receptor type 1 , PTP1B
Cellular Localization
Endoplasmic reticulum membrane
Background
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]
Database
Research Area
DATA IMAGES
![Anti-PTP1B antibody [4F8F11] used in Flow cytometry (FACS). GTX04099](/templates/default/js/lightbox/images/loading.gif "Anti-PTP1B antibody [4F8F11] used in Flow cytometry (FACS). GTX04099")
|
GTX04099 FACS Image
FACS analysis of Jurkat cells using GTX04099 PTP1B antibody [4F8F11].
Green : Primary antibody
Red : Negative control
|
|
|
GTX04099 IHC-P Image
IHC-P analysis of human bladder cancer tissues using GTX04099 PTP1B antibody [4F8F11].
|
|
|
GTX04099 WB Image
WB analysis of PTP1B (AA: 40-246), human IgG Fc tag transfected HEK293 lysates using GTX04099 PTP1B antibody [4F8F11].
Lane 1 : HEK293
Lane 2 : PTP1B (AA: 40-246), human IgG Fc tag transfected HEK293
|
|
|
GTX04099 ICC/IF Image
ICC/IF analysis of Hela cells using GTX04099 PTP1B antibody [4F8F11].
Green : Primary antibody
Blue : DRAQ5 fluorescent DNA dye
Red : Actin filament
|
|
|
GTX04099 WB Image
WB analysis of human PTP1B (AA: 40-246) recombinant protein using GTX04099 PTP1B antibody [4F8F11].
|
|
|
GTX04099 ELISA Image
ELISA analysis of antigen proteins using GTX04099 PTP1B antibody [4F8F11].
Black line : Control Antigen (100 ng)
Purple line : Antigen (10 ng)
Blue line : Antigen (50 ng)
Red line : Antigen (100 ng)
|
|
|
GTX04099 WB Image
WB analysis of various cell lysates using GTX04099 PTP1B antibody [4F8F11].
Lane 1 : Jurkat cell lysates
Lane 2 : MCF-7 cell lysates
|
REFERENCE
There are currently no references for PTP1B antibody [4F8F11] (GTX04099). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PTP1B antibody [4F8F11] (GTX04099). Be the first to share your experience with this product.
![Anti-PTP1B antibody [4F8F11] used in Flow cytometry (FACS). GTX04099](/upload/website/prouct_img/small/GTX04099/GTX04099_20221104_FACS_22110318_687.webp "Anti-PTP1B antibody [4F8F11] used in Flow cytometry (FACS). GTX04099")
![Anti-PTP1B antibody [4F8F11] used in IHC (Paraffin sections) (IHC-P). GTX04099](/upload/website/prouct_img/small/GTX04099/GTX04099_20221104_IHC-P_22110318_757.webp "Anti-PTP1B antibody [4F8F11] used in IHC (Paraffin sections) (IHC-P). GTX04099")
![Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099](/upload/website/prouct_img/small/GTX04099/GTX04099_20221104_WB_1_22110318_491.webp "Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099")
![Anti-PTP1B antibody [4F8F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04099](/upload/website/prouct_img/small/GTX04099/GTX04099_20221104_ICCIF_22110318_356.webp "Anti-PTP1B antibody [4F8F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04099")
![Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099](/upload/website/prouct_img/small/GTX04099/GTX04099_20221104_WB_22110318_204.webp "Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099")
![Anti-PTP1B antibody [4F8F11] used in ELISA (ELISA). GTX04099](/upload/website/prouct_img/small/GTX04099/GTX04099_20221104_ELISA_22110318_449.webp "Anti-PTP1B antibody [4F8F11] used in ELISA (ELISA). GTX04099")
![Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099](/upload/website/prouct_img/small/GTX04099/GTX04099_20221104_WB_2_22110318_789.webp "Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099")
![Anti-PTP1B antibody [4F8F11] used in Flow cytometry (FACS). GTX04099](/upload/website/prouct_img/normal/GTX04099/GTX04099_20221104_FACS_22110318_687.webp "Anti-PTP1B antibody [4F8F11] used in Flow cytometry (FACS). GTX04099")
![Anti-PTP1B antibody [4F8F11] used in IHC (Paraffin sections) (IHC-P). GTX04099](/upload/website/prouct_img/normal/GTX04099/GTX04099_20221104_IHC-P_22110318_757.webp "Anti-PTP1B antibody [4F8F11] used in IHC (Paraffin sections) (IHC-P). GTX04099")
![Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099](/upload/website/prouct_img/normal/GTX04099/GTX04099_20221104_WB_1_22110318_491.webp "Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099")
![Anti-PTP1B antibody [4F8F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04099](/upload/website/prouct_img/normal/GTX04099/GTX04099_20221104_ICCIF_22110318_356.webp "Anti-PTP1B antibody [4F8F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04099")
![Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099](/upload/website/prouct_img/normal/GTX04099/GTX04099_20221104_WB_22110318_204.webp "Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099")
![Anti-PTP1B antibody [4F8F11] used in ELISA (ELISA). GTX04099](/upload/website/prouct_img/normal/GTX04099/GTX04099_20221104_ELISA_22110318_449.webp "Anti-PTP1B antibody [4F8F11] used in ELISA (ELISA). GTX04099")
![Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099](/upload/website/prouct_img/normal/GTX04099/GTX04099_20221104_WB_2_22110318_789.webp "Anti-PTP1B antibody [4F8F11] used in Western Blot (WB). GTX04099")