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PTP1B antibody

Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX04098
Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX04098
Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX04098
Anti-PTP1B antibody used in Western Blot (WB). GTX04098
Anti-PTP1B antibody used in Western Blot (WB). GTX04098
Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX04098
Anti-PTP1B antibody used in Flow cytometry (FACS). GTX04098
Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX04098
Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX04098
Anti-PTP1B antibody used in IHC (Frozen sections) (IHC-Fr). GTX04098
Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX04098

Cat. No. GTX04098

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($429)
See all PTP1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml
IHC-Fr 0.5-1μg/ml
FACS 1-3μg/10⁶ cells

Note :

IHC-P
Antigen retrieval by heat
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4

Preservative

0.05 mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PTP1B (4-30aa EKEFEQIDKSGSWAAIYQDIRHEASDF), different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase non-receptor type 1 , PTP1B

Cellular Localization

Endoplasmic reticulum membrane

Background

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX04098

GTX04098 IHC-P Image

IHC-P analysis of mouse testis tissues using GTX04098 PTP1B antibody.
Dilution : 1 μg/ml
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution) for 20 mins

Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX04098

GTX04098 IHC-P Image

IHC-P analysis of rat spleen tissues using GTX04098 PTP1B antibody.
Dilution : 1 μg/ml
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution) for 20 mins

Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX04098

GTX04098 IHC-P Image

IHC-P analysis of mouse spleen tissues using GTX04098 PTP1B antibody.
Dilution : 1 μg/ml
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution) for 20 mins

Anti-PTP1B antibody used in Western Blot (WB). GTX04098

GTX04098 WB Image

WB analysis of various sample lysates using GTX04098 PTP1B antibody.
Lane 1 : human placenta tissue lysates
Lane 2 : MCF-7 whole cell lysates
Lane 3 : Caco-2 whole cell lysates
Lane 4 : K562 whole cell lysates
Lane 5 : U2OS whole cell lysates
Lane 6 : PC-3 whole cell lysates
Lane 7 : HL-60 whole cell lysates
Lane 8 : A549 whole cell lysates
Dilution : 0.5 μg/mL
Loading : 50 μg

Anti-PTP1B antibody used in Western Blot (WB). GTX04098

GTX04098 WB Image

WB analysis of various sample lysates using GTX04098 PTP1B antibody.
Lane 1 : rat kidney tissue lysate
Lane 2 : mouse kidney tissue lysate
Dilution : 0.5 μg/mL
Loading : 50 μg

Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX04098

GTX04098 IHC-P Image

IHC-P analysis of human tonsil tissues using GTX04098 PTP1B antibody.
Dilution : 1 μg/ml
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution) for 20 mins

Anti-PTP1B antibody used in Flow cytometry (FACS). GTX04098

GTX04098 FACS Image

FACS analysis of U20S cells using GTX04098 PTP1B antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Antibody amount : 1 μg/1x10⁶ cells

Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX04098

GTX04098 IHC-P Image

IHC-P analysis of human tonsil tissues using GTX04098 PTP1B antibody.
Dilution : 1 μg/ml
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution) for 20 mins

Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX04098

GTX04098 IHC-P Image

IHC-P analysis of human placenta tissues using GTX04098 PTP1B antibody.
Dilution : 1 μg/ml
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution) for 20 mins

Anti-PTP1B antibody used in IHC (Frozen sections) (IHC-Fr). GTX04098

GTX04098 IHC-Fr Image

IHC-Fr analysis of human placenta tissues using GTX04098 PTP1B antibody.
Dilution : 1 μg/ml

Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX04098

GTX04098 IHC-P Image

IHC-P analysis of human mammary cancer tissues using GTX04098 PTP1B antibody.
Dilution : 1 μg/ml
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution) for 20 mins

REFERENCE

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REVIEW

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Package List Price ($)
$ 429