GeneTex
United States (US)

PTPN12 antibody [N1N2], N-term

Genetex PTPN12 542 1 in 20000 400X  small 5.4.11_19011720_150.jpg
GTX102542_40387_IHC_R_19011506_598.jpg
GTX102542_40387_IFA_19011506_650.jpg
GTX102542_40387_WB_19011506_998.jpg
GTX102542_40387_IHC_19011506_931.jpg

Cat No. GTX102542

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2

Predict Reactivity

Mouse, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PTPN12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Tyrosine Phosphatase, Non-Receptor Type 12,Ptp-Pest,Ptpg1,Ptpn12

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may regulate protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of the oncogene c-ABL and thus may play a role in oncogenesis. This PTP was also shown to interact with, and dephosphorylate, various products related to cytoskeletal structure and cell adhesion, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin. This suggests it has a regulatory role in controlling cell shape and mobility. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

DATA IMAGES
Genetex PTPN12 542 1 in 20000 400X  small 5.4.11_19011720_150.jpg

GTX102542 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using PTPN12 antibody (GTX102542)

GTX102542_40387_IHC_R_19011506_598.jpg

GTX102542 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using PTPN12(GTX102542) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102542_40387_IFA_19011506_650.jpg

GTX102542 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PTPN12(GTX102542) antibody at 1:200 dilution.

GTX102542_40387_WB_19011506_998.jpg

GTX102542 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
5% SDS PAGE
GTX102542 diluted at 1:5000

GTX102542_40387_IHC_19011506_931.jpg

GTX102542 IHC-P Image

Immunohistochemical analysis of paraffin-embedded D54 xenograft, using PTPN12(GTX102542) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169