APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Mouse, Bovine, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.93 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human PTPN12. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10730637
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein tyrosine phosphatase non-receptor type 12 , PTP-PEST , PTPG1
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may regulate protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of the oncogene c-ABL and thus may play a role in oncogenesis. This PTP was also shown to interact with, and dephosphorylate, various products related to cytoskeletal structure and cell adhesion, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin. This suggests it has a regulatory role in controlling cell shape and mobility. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-PTPN12 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102542](/templates/default/js/lightbox/images/loading.gif "Anti-PTPN12 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102542")
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GTX102542 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using PTPN12 antibody (GTX102542)
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GTX102542 IHC-P Image
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using PTPN12(GTX102542) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102542 WB Image
Sample (30 ug of whole cell lysate)
A: HeLa
5% SDS PAGE
GTX102542 diluted at 1:5000
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GTX102542 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using PTPN12(GTX102542) antibody at 1:200 dilution.
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GTX102542 IHC-P Image
Immunohistochemical analysis of paraffin-embedded D54 xenograft, using PTPN12(GTX102542) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for PTPN12 antibody [N1N2], N-term (GTX102542). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PTPN12 antibody [N1N2], N-term (GTX102542). Be the first to share your experience with this product.
![Anti-PTPN12 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102542](/upload/website/prouct_img/small/GTX102542/Genetex PTPN12 542 1 in 20000 400X small 5.4.11_w_23060100_937.webp "Anti-PTPN12 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102542")
![Anti-PTPN12 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102542](/upload/website/prouct_img/small/GTX102542/GTX102542_40387_IHC_R_w_23060100_675.webp "Anti-PTPN12 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102542")
![Anti-PTPN12 antibody [N1N2], N-term used in Western Blot (WB). GTX102542](/upload/website/prouct_img/small/GTX102542/GTX102542_40387_WB_w_23060100_857.webp "Anti-PTPN12 antibody [N1N2], N-term used in Western Blot (WB). GTX102542")
![Anti-PTPN12 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102542](/upload/website/prouct_img/small/GTX102542/GTX102542_40387_IFA_w_23060100_840.webp "Anti-PTPN12 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102542")
![Anti-PTPN12 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102542](/upload/website/prouct_img/small/GTX102542/GTX102542_40387_IHC_w_23060100_749.webp "Anti-PTPN12 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102542")
![Anti-PTPN12 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102542](/upload/website/prouct_img/normal/GTX102542/Genetex PTPN12 542 1 in 20000 400X small 5.4.11_w_23060100_937.webp "Anti-PTPN12 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102542")
![Anti-PTPN12 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102542](/upload/website/prouct_img/normal/GTX102542/GTX102542_40387_IHC_R_w_23060100_675.webp "Anti-PTPN12 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102542")
![Anti-PTPN12 antibody [N1N2], N-term used in Western Blot (WB). GTX102542](/upload/website/prouct_img/normal/GTX102542/GTX102542_40387_WB_w_23060100_857.webp "Anti-PTPN12 antibody [N1N2], N-term used in Western Blot (WB). GTX102542")
![Anti-PTPN12 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102542](/upload/website/prouct_img/normal/GTX102542/GTX102542_40387_IFA_w_23060100_840.webp "Anti-PTPN12 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102542")
![Anti-PTPN12 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102542](/upload/website/prouct_img/normal/GTX102542/GTX102542_40387_IHC_w_23060100_749.webp "Anti-PTPN12 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102542")