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PTPRE antibody [2A9]

Anti-PTPRE antibody [2A9] used in IHC (Paraffin sections) (IHC-P). GTX83761
Anti-PTPRE antibody [2A9] used in Flow cytometry (FACS). GTX83761
Anti-PTPRE antibody [2A9] used in IHC (Paraffin sections) (IHC-P). GTX83761
Anti-PTPRE antibody [2A9] used in Western Blot (WB). GTX83761
Anti-PTPRE antibody [2A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83761
Anti-PTPRE antibody [2A9] used in IHC (Paraffin sections) (IHC-P). GTX83761

Cat. No. GTX83761

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2A9

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all PTPRE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PTPRE (NP_006495) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase receptor type E , HPTPE , PTPE , R-PTP-EPSILON

Background

Isoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function. Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake. Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca(2+).

Database

Research Area

DATA IMAGES

Anti-PTPRE antibody [2A9] used in IHC (Paraffin sections) (IHC-P). GTX83761

GTX83761 IHC-P Image

IHC-P analysis of human prostate tissue using GTX83761 PTPRE antibody [2A9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PTPRE antibody [2A9] used in Flow cytometry (FACS). GTX83761

GTX83761 FACS Image

FACS analysis of HEK293T cells transfected with either PTPRE plasmid(Red) or empty vector control plasmid(Blue) using GTX83761 PTPRE antibody [2A9].

Anti-PTPRE antibody [2A9] used in IHC (Paraffin sections) (IHC-P). GTX83761

GTX83761 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX83761 PTPRE antibody [2A9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PTPRE antibody [2A9] used in Western Blot (WB). GTX83761

GTX83761 WB Image

WB analysis of HeLa and MCF-7 cell lysate using GTX83761 PTPRE antibody [2A9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-PTPRE antibody [2A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83761

GTX83761 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PTPRE plasmid using GTX83761 PTPRE antibody [2A9].

Anti-PTPRE antibody [2A9] used in IHC (Paraffin sections) (IHC-P). GTX83761

GTX83761 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX83761 PTPRE antibody [2A9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519