GeneTex

  • Country / Location Selection

United States (US)

PTS antibody [N1C3]

KO/KD Overexpression
Anti-PTS antibody [N1C3] used in Western Blot (WB). GTX110549
Anti-PTS antibody [N1C3] used in Western Blot (WB). GTX110549
Anti-PTS antibody [N1C3] used in Western Blot (WB). GTX110549
Anti-PTS antibody [N1C3] used in Western Blot (WB). GTX110549
Anti-PTS antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110549
Anti-PTS antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110549
Anti-PTS antibody [N1C3] used in Western Blot (WB). GTX110549

Cat. No. GTX110549

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PTS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

Mouse stomach , H1299 , HeLa , PTS-transfected 293T

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human PTS Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951548

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

6-pyruvoyltetrahydropterin synthase , PTPS

Background

The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PTS antibody [N1C3] used in Western Blot (WB). GTX110549

GTX110549 WB Image

KO/KD

Wild-type (WT) and PTS knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PTS antibody [N1C3] (GTX110549) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PTS antibody [N1C3] used in Western Blot (WB). GTX110549

GTX110549 WB Image

Sample (50 ug of whole cell lysate)
A: mouse stomach
15% SDS PAGE
GTX110549 diluted at 1:1000

Anti-PTS antibody [N1C3] used in Western Blot (WB). GTX110549

GTX110549 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
15% SDS PAGE
GTX110549 diluted at 1:1000

Anti-PTS antibody [N1C3] used in Western Blot (WB). GTX110549

GTX110549 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PTS antibody [N1C3] (GTX110549) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PTS antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110549

GTX110549 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PTS(GTX110549) antibody at 1:100 dilution.

Anti-PTS antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110549

GTX110549 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using PTS(GTX110549) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PTS antibody [N1C3] used in Western Blot (WB). GTX110549

GTX110549 WB Image

The data was published in the journal Hum Mol Genet in 2016.PMID: 27798097

REFERENCE

Application Reference

Ishikawa T et al. Hum Mol Genet 2016; (Epub) Genetic and pharmacological correction of aberrant * synthesis using patient iPSCs with BH4 metabolism disorders.
Application : WB
Submit a Reference

REVIEW

There are currently no reviews for PTS antibody [N1C3] (GTX110549). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169