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PU.1 antibody [5C7]

Anti-PU.1 antibody [5C7] used in Flow cytometry (FACS). GTX60620
Anti-PU.1 antibody [5C7] used in Western Blot (WB). GTX60620
Anti-PU.1 antibody [5C7] used in ELISA (ELISA). GTX60620

Cat. No. GTX60620

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5C7

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all PU.1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PU.1 (AA: 124-271) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Spi-1 proto-oncogene , OF , PU.1 , SFPI1 , SPI-1 , SPI-A

Cellular Localization

Nucleus

Background

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PU.1 antibody [5C7] used in Flow cytometry (FACS). GTX60620

GTX60620 FACS Image

FACS analysis of HeLa cells using GTX60620 PU.1 antibody [5C7].
Green : PU.1
Red : negative control

Anti-PU.1 antibody [5C7] used in Western Blot (WB). GTX60620

GTX60620 WB Image

WB analysis of HEK293 (1) and PU.1 (AA: 124-271)-hIgGFc transfected HEK293 (2) cell lysate using GTX60620 PU.1 antibody [5C7].

Anti-PU.1 antibody [5C7] used in ELISA (ELISA). GTX60620

GTX60620 ELISA Image

ELISA analysis of antigen using GTX60620 PU.1 antibody [5C7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409