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PU.1 antibody, Internal

Anti-PU.1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88620
Anti-PU.1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88620
Anti-PU.1 antibody, Internal used in Western Blot (WB). GTX88620

Cat. No. GTX88620

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all PU.1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.03-0.1μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88620-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2).

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DLYQRQTHEYY, from the internal region of the protein sequence according to NP_001074016.1; NP_003111.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Spi-1 proto-oncogene , OF , PU.1 , SFPI1 , SPI-1 , SPI-A

Cellular Localization

Nucleus

Background

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PU.1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88620

GTX88620 IHC-P Image

IHC-P analysis of human colon using GTX88620 PU.1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-PU.1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88620

GTX88620 IHC-P Image

IHC-P analysis of human spleen using GTX88620 PU.1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-PU.1 antibody, Internal used in Western Blot (WB). GTX88620

GTX88620 WB Image

WB analysis of Daudi lysate using GTX88620 PU.1 antibody, Internal.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399