GeneTex
United States (US)

PU.1 antibody, Internal

GTX88620_2127_WB_18122102_693.jpg
GTX88620_852_IHC-P_18122102_746.jpg
GTX88620_851_IHC-P_18122102_730.jpg

Cat No. GTX88620

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.03-0.1μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88620-PEP).

Specificity/Sensitivity

This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2).

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DLYQRQTHEYY, from the internal region of the protein sequence according to NP_001074016.1; NP_003111.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Spi-1 Proto-Oncogene,Of,Pu.1,Sfpi1,Spi-1,Spi-A,Spi1

Background

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88620_2127_WB_18122102_693.jpg

GTX88620 WB Image

WB analysis of Daudi lysate using GTX88620 PU.1 antibody, Internal.
Dilution : 0.03µg/ml
Loading : 35µg protein in RIPA buffer

GTX88620_852_IHC-P_18122102_746.jpg

GTX88620 IHC-P Image

IHC-P analysis of human spleen using GTX88620 PU.1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88620_851_IHC-P_18122102_730.jpg

GTX88620 IHC-P Image

IHC-P analysis of human colon using GTX88620 PU.1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

Package List Price ($)
$ 329