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PU.1 antibody

Anti-PU.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66691
Anti-PU.1 antibody used in Western Blot (WB). GTX66691
Anti-PU.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66691
Anti-PU.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66691
Anti-PU.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66691
Anti-PU.1 antibody used in Western Blot (WB). GTX66691

Cat No. GTX66691

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Rat
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 180-230 of Human PU.1.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Spi-1 proto-oncogene , OF , PU.1 , SFPI1 , SPI-1 , SPI-A

Cellular Localization

Nucleus

Background

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PU.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66691

GTX66691 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX66691 PU.1 antibody.
Left : Primary antibody
Right : Negative control
Dilution : 1:50

Anti-PU.1 antibody used in Western Blot (WB). GTX66691

GTX66691 WB Image

WB analysis of various samples using GTX66691 PU.1 antibody.
Lane1 : HepG2 whole cell lysate(40ug)
Lane2 : SGC7901 whole cell lysate(40ug)
Lane3 : L02 whole cell lysate(40ug)
Dilution : 1:500

Anti-PU.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66691

GTX66691 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX66691 PU.1 antibody.
Left : Primary antibody
Right : Negative control
Dilution : 1:50

Anti-PU.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66691

GTX66691 IHC-P Image

IHC-P analysis of human tonsil carcinoma tissue using GTX66691 PU.1 antibody.
Left : Primary antibody
Right : Negative control
Dilution : 1:50

Anti-PU.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66691

GTX66691 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX66691 PU.1 antibody.
Left : Primary antibody
Right : Negative control
Dilution : 1:50

Anti-PU.1 antibody used in Western Blot (WB). GTX66691

GTX66691 WB Image

WB analysis of various samples using GTX66691 PU.1 antibody.
Lane1 : JURKAT whole cell lysate(37ug)
Lane2 : THP-1 whole cell lysate(37ug)
Lane3 : The blood cell tissue lysate of Rat(36ug)
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 349