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PUF60 antibody, C-term

Anti-PUF60 antibody, C-term used in Western Blot (WB). GTX89681

Cat. No. GTX89681

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all PUF60 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89681-PEP).

Product Note

This antibody is expected to recognise all reported human isoforms (as represented by NP_510965.1; NP_055096.2; NP_001129505.1)

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-YDQERFDNSDLSA, from the C Terminus of the protein sequence according to NP_510965.1; NP_055096.2; NP_001129505.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(U) binding splicing factor 60 , FIR , RoBPI , SIAHBP1 , VRJS

Cellular Localization

Nucleus

Background

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-PUF60 antibody, C-term used in Western Blot (WB). GTX89681

GTX89681 WB Image

WB analysis of HEK293 lysate using GTX89681 PUF60 antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399