APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , mouse brain , rat brain
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PUF60. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888174
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
poly(U) binding splicing factor 60 , FIR , RoBPI , SIAHBP1 , VRJS
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX629885 WB Image
PUF60 antibody [GT3510] detects PUF60 protein by Western blot analysis. A. 50 μg rat brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [GT3510] (GTX629885) dilution: 1:1000
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GTX629885 ICC/IF Image
PUF60 antibody [GT3510] detects PUF60 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PUF60 protein stained by PUF60 antibody [GT3510] (GTX629885) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX629885 WB Image
PUF60 antibody [GT3510] detects PUF60 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE PUF60 antibody [GT3510] (GTX629885) dilution: 1:5000
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GTX629885 IHC-P Image
PUF60 antibody [GT3510] detects PUF60 protein at on SAS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SAS xenograft. PUF60 antibody [GT3510] (GTX629885) dilution: 1:100.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX629885 WB Image
PUF60 antibody [GT3510] detects PUF60 protein by Western blot analysis. A. 50 μg mouse brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [GT3510] (GTX629885) dilution: 1:1000
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GTX629885 IHC-P Image
PUF60 antibody [GT3510] detects PUF60 protein at on AGS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded gastric carcinoma. PUF60 antibody [GT3510] (GTX629885) dilution: 1:100.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
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REVIEW
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