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PUF60 antibody [GT677]

Anti-PUF60 antibody [GT677] used in Western Blot (WB). GTX629886
Anti-PUF60 antibody [GT677] used in IHC (Paraffin sections) (IHC-P). GTX629886
Anti-PUF60 antibody [GT677] used in Western Blot (WB). GTX629886
Anti-PUF60 antibody [GT677] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629886
Anti-PUF60 antibody [GT677] used in Western Blot (WB). GTX629886

Cat. No. GTX629886

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT677

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PUF60 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human PUF60

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888175

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(U) binding splicing factor 60 , FIR , RoBPI , SIAHBP1 , VRJS

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PUF60 antibody [GT677] used in Western Blot (WB). GTX629886

GTX629886 WB Image

PUF60 antibody [GT677] detects PUF60 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
7.5% SDS-PAGE
PUF60 antibody [GT677] (GTX629886) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PUF60 antibody [GT677] used in IHC (Paraffin sections) (IHC-P). GTX629886

GTX629886 IHC-P Image

PUF60 antibody [GT677] detects PUF60 protein at on gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded AGS xenograft.
PUF60 antibody [GT677] (GTX629886) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PUF60 antibody [GT677] used in Western Blot (WB). GTX629886

GTX629886 WB Image

PUF60 antibody [GT677] detects PUF60 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5% SDS-PAGE
PUF60 antibody [GT677] (GTX629886) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PUF60 antibody [GT677] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629886

GTX629886 ICC/IF Image

PUF60 antibody [GT677]PUF60 antibody [GT677] detects PUF60 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PUF60 protein stained by PUF60 antibody [GT677] (GTX629886) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-PUF60 antibody [GT677] used in Western Blot (WB). GTX629886

GTX629886 WB Image

PUF60 antibody [GT677] detects PUF60 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5% SDS-PAGE
PUF60 antibody [GT677] (GTX629886) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations