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PUF60 antibody

Anti-PUF60 antibody used in Western Blot (WB). GTX102051
Anti-PUF60 antibody used in IHC (Paraffin sections) (IHC-P). GTX102051
Anti-PUF60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102051
Anti-PUF60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102051

Cat No. GTX102051

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PUF60. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

poly(U) binding splicing factor 60 , FIR , RoBPI , SIAHBP1 , VRJS

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PUF60 antibody used in Western Blot (WB). GTX102051

GTX102051 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX102051 diluted at 1:1000

Anti-PUF60 antibody used in IHC (Paraffin sections) (IHC-P). GTX102051

GTX102051 IHC-P Image

PUF60 antibody detects PUF60 protein at nucleus on U251 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U251 xenograft.
PUF60 antibody (GTX102051) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PUF60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102051

GTX102051 ICC/IF Image

PUF60 antibody detects PUF60 protein at nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PUF60 protein stained by PUF60 antibody (GTX102051) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-PUF60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102051

GTX102051 ICC/IF Image

PUF60 antibody detects PUF60 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PUF60 protein stained by PUF60 antibody (GTX102051) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169