GeneTex
United States (US)

PUF60 antibody

GTX102051_40632_WB_19011506_388.jpg
GTX102051_41066_IHC_19011506_230.jpg
GTX102051_41066_IFA_2_19011506_326.jpg
GTX102051_41066_20150525_IFA_19011506_396.jpg

Cat No. GTX102051

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PUF60. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FIR antibody, FLJ31379 antibody, RoBPI antibody, SIAHBP1 antibody, PUF60 antibody, poly(U)-binding-splicing factor PUF60 antibody, Ro ribonucleoprotein-binding protein 1 antibody, ro-binding protein 1 antibody, siah-BP1 antibody, FBP interacting repressor antibody, roBP1 antibody, poly-U binding splicing factor PUF60 antibody, Ro ribonucleoprotein binding protein 1 antibody, siah binding protein 1 antibody, 60 kDa poly(U)-binding-splicing factor antibody, siah-binding protein 1 antibody, fuse-binding protein-interacting repressor antibody, pyrimidine tract binding splicing factor antibody, FBP-interacting repressor antibody, poly-U binding splicing factor 60KDa antibody

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102051_40632_WB_19011506_388.jpg

GTX102051 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX102051 diluted at 1:1000

GTX102051_41066_IHC_19011506_230.jpg

GTX102051 IHC-P Image

PUF60 antibody detects PUF60 protein at nucleus on U251 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U251 xenograft.
PUF60 antibody (GTX102051) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102051_41066_IFA_2_19011506_326.jpg

GTX102051 ICC/IF Image

PUF60 antibody detects PUF60 protein at nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PUF60 protein stained by PUF60 antibody (GTX102051) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX102051_41066_20150525_IFA_19011506_396.jpg

GTX102051 ICC/IF Image

PUF60 antibody detects PUF60 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PUF60 protein stained by PUF60 antibody (GTX102051) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169