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PUF60 antibody

Anti-PUF60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102052
Anti-PUF60 antibody used in IHC (Paraffin sections) (IHC-P). GTX102052
Anti-PUF60 antibody used in Western Blot (WB). GTX102052
Anti-PUF60 antibody used in IHC (Whole mount) (IHC-Wm). GTX102052
Anti-PUF60 antibody used in IHC (Paraffin sections) (IHC-P). GTX102052
Anti-PUF60 antibody used in Western Blot (WB). GTX102052

Cat. No. GTX102052

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all PUF60 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PUF60. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951551

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(U) binding splicing factor 60 , FIR , RoBPI , SIAHBP1 , VRJS

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PUF60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102052

GTX102052 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using PUF60(GTX102052) antibody at 1:500 dilution.

Anti-PUF60 antibody used in IHC (Paraffin sections) (IHC-P). GTX102052

GTX102052 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human oral tissue, using PUF60(GTX102052) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PUF60 antibody used in Western Blot (WB). GTX102052

GTX102052 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
B:MOLT4(GTX27912)
C: Raji (GTX27908)
7.5% SDS PAGE
GTX102052 diluted at 1:500

Anti-PUF60 antibody used in IHC (Whole mount) (IHC-Wm). GTX102052

GTX102052 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using PUF60 antibody (GTX102052) at 1:200 dilution.

Anti-PUF60 antibody used in IHC (Paraffin sections) (IHC-P). GTX102052

GTX102052 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using PUF60 antibody (GTX102052) at 1:300 dilution.

Anti-PUF60 antibody used in Western Blot (WB). GTX102052

GTX102052 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish brain
7.5% SDS PAGE
GTX102052 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169