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PUF60 antibody

Anti-PUF60 antibody used in Western Blot (WB). GTX33446
Anti-PUF60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33446
Anti-PUF60 antibody used in IHC (Paraffin sections) (IHC-P). GTX33446
Anti-PUF60 antibody used in Immunoprecipitation (IP). GTX33446

Cat No. GTX33446

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:100
IHC-P 1:50-1:200
IP 1:20-1:50
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 243-542 of human PUF60 (NP_055096.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

poly(U) binding splicing factor 60 , FIR , RoBPI , SIAHBP1 , VRJS

Cellular Localization

Nucleus

Background

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-PUF60 antibody used in Western Blot (WB). GTX33446

GTX33446 WB Image

WB analysis of various sample lysates using GTX33446 PUF60 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-PUF60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33446

GTX33446 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33446 PUF60 antibody.
Blue : DAPI

Anti-PUF60 antibody used in IHC (Paraffin sections) (IHC-P). GTX33446

GTX33446 IHC-P Image

IHC-P analysis of human well-differentiated squamous skin carcinoma tissue using GTX33446 PUF60 antibody.
Dilution : 1:100

Anti-PUF60 antibody used in Immunoprecipitation (IP). GTX33446

GTX33446 IP Image

IP analysis of A431 cells using GTX33446 PUF60 antibody.
IP reaction : 150μg extracts / 3μg antibody antibody
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf