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PUMA antibody [10D4G7]

Anti-PUMA antibody [10D4G7] used in Western Blot (WB). GTX84989

Cat. No. GTX84989

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10D4G7

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human, Rat
Package
100 μg ($429)
See all PUMA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2.5 to 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Positive Control

K562 Cell Lysate(GTX27911) , PUMA Recombinant Protein(GTX48024-PRO)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 binding component 3 , JFY-1 , JFY1 , PUMA

Cellular Localization

Mitochondrion

Background

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Database

Research Area

DATA IMAGES

Anti-PUMA antibody [10D4G7] used in Western Blot (WB). GTX84989

GTX84989 WB Image

WB analysis of K562 cell lysate using GTX84989 PUMA antibody [10D4G7].
Working concentration : (A) 2.5 and (B) 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 429