GeneTex
United States (US)

PUMA antibody [C2C3], C-term

Orthogonal
GTX109675_40030_WB_HCT116 cisplatin_19011720_402.jpg
GTX109675_40030_IHC_19011507_284.jpg
GTX109675_40030_IP_19011507_774.jpg
GTX109675_40030_WB_19011507_821.jpg

Cat No. GTX109675

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

K562 , THP-1 , HCT116 control 0hr , HCT116 treated 30uM cisplatin 24hr
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PUMA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ42994 antibody, JFY1 antibody, PUMA antibody, BBC3 antibody, p53 up-regulated modulator of apoptosis antibody, bcl-2-binding component 3 antibody, BCL2 binding component 3 antibody

Cellular Localization

Mitochondrion

Research Area

DATA IMAGES
GTX109675_40030_WB_HCT116 cisplatin_19011720_402.jpg

GTX109675 WB Image

Orthogonal

Sample (30 ug of whole cell lysate)
A: HCT116 cells with mock treatment for 0hr
B: HCT116 cells with 30uM cisplatin treatment for 24hr
15% SDS PAGE
GTX109675 diluted at 1:1000

GTX109675_40030_IHC_19011507_284.jpg

GTX109675 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using PUMA(GTX109675) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109675_40030_IP_19011507_774.jpg

GTX109675 IP Image

PUMA antibody [C2C3], C-term immunoprecipitates BCL2 binding component 3 protein in IP experiments.
IP samples: HCT-116 whole cell extract treat with 30uM cisplatin for 24 hr
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of BCL2 binding component 3 protein by 4 μg PUMA antibody [C2C3], C-term (GTX109675)
15 % SDS-PAGE
The immunoprecipitated BCL2 binding component 3 protein was detected by PUMA antibody [C2C3], C-term (GTX109675) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX109675_40030_WB_19011507_821.jpg

GTX109675 WB Image

PUMA antibody detects PUMA protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12 % SDS-PAGE, and blotted with PUMA antibody (GTX109675) diluted by 1:500

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169